Experimental breeding sites simulating natural conditions were used to evaluate the efficacy of 2 formulations of Bacillus sphaericus (strain 2362) against Anopheles albimanus and culicine (mostly Culex coronator and Cx. quinquefasciatus) mosquito larvae of southern Mexico. Three doses of each formulation were used in a first field trial: 2, 3 and 4 g/m2 (granular) or 2, 3 and 4 ml/m2 (liquid); and in a second field trial: 0.125, 0.24 and 0.5 g/m2 (granular) or 0.125, 0.25 and 0.5 ml/m2 (liquid). The optimum concentrations of each formulation for effective control of larval populations over periods of 3-4 months were 0.125 ml/m2 of liquid product for Culex spp. and 2 g/m2 of granular product for An. albimanus (ca. 70% mean reduction).